This study explored the potential of combining platelet-rich fibrin (PRF) with skin-derived mesenchymal stem cells (SMSCs) to enhance wound healing through increased growth factor production. Using 16 New Zealand white rabbits, PRF and SMSCs were isolated and co-cultured for up to five days. SEM analysis showed that by day five, SMSCs adhered and spread uniformly on the PRF surface, displaying elongated morphology and good biocompatibility. ELISA results indicated significantly higher levels of PDGF, IGF, VEGF, and TGF-β in PRF with SMSCs compared to PRF alone (p<0.01). These findings demonstrate that PRF supports SMSC proliferation and boosts growth factor release, suggesting its strong potential as a biological scaffold for tissue regeneration in veterinary medicine.
Tim Infografik:
- Igo Syaiful Ihsan, drh., M.Si
- Nora Ertanti
- Deya Kersari